Previous studies have revealed some genetic interactions between residues within the Pol II TL, suggesting a functional network controlling transcription activity and potentially genetically separable steps in TL function. However, detailed mechanisms that control the TL and communicate to it within these enzymes remain to be elucidated. It has been shown that substitutions of TL residues alter key aspects of transcription. Multi-subunit RNA polymerases (msRNAPs) require a highly conserved, flexible “trigger loop” (TL) domain to promote fast and accurate transcription (Figure 3B).
Functional dissection of RNA polymerase active sites by deep mutational scanningīingbing Duan, Kaplan lab, Department of Biological SciencesĭNA-dependent RNA transcription is essential for all kingdoms of life. Poster Contest Guidelines and Judging Criteriaġ.
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